我们通过控制水解TEOS的方法获得了核壳结构的SiO2包裹的氧化铁复合磁性纳米粒子MNP@SiO2,复合粒子的平均粒径为30nm,并在SiO2的包覆层负载荧光分子FITC.我们进一步将尼妥珠单抗h-R3标记于粒子表面.体外细胞实验结果表明, 尼妥珠单抗h-R3标记的氧化铁复合磁性纳米粒子对于EGFR过表达的肿瘤细胞A431具有明显的靶向性和细胞毒性,预示着该粒子可作为EGFR过表达细胞的靶向探针.
Ming Ma, Yu Zhang, Haiwei Gong, Fangqiu Li, and Ning Gu, Silica-Coated Magnetite Nanoparticles Labeled by Nimotuzumab, A Humanised Monoclonal Antibody to Epidermal Growth Factor Receptor: Preparations, Specific Targeting and Bioimaging, J. Nanosci. Nanotechnol. 13, 6541-6545 (2013)
Fig. 1. TEM image of MNP@SiO2 (A) and Hydrodynamics size distributions of MNP@SiO2 and MNP@SiO2-mAbh−R3
Fig. 4. The LSCM images of A431 cells treated with FITC doped MNP@SiO2-mAbh−R3 (A) and MNP@SiO2-BSA (B) for 2 h. The concentrations of MNP@SiO2-mAbh−R3 and MNP@SiO2-BSA in culture media were 10 _g Fe/mL. The left is LSCM image, and the right is the merged image of LSCM and the phase contrast images.
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