本文研究了纳米氧化铁对绿豆植物各种生理参数的影响,包括对种子萌发、根系活力、过氧化物酶、过氧化氢酶、超氧化歧化酶、叶绿素、可溶性蛋白质和丙二醛含量几个参数的影响。结果表明:纳米氧化铁浓度越小,对绿豆根系活力影响越大,能够促进根系生长。同时,丙二醛(MDA)含量随浓度增大而降低;可溶性蛋白质含量随浓度增大而增大;超氧化物歧化酶(SOD)活性减小;过氧化物酶(POD) 过氧化氢酶(CAT)活性随着浓度增大,影响变小;纳米颗粒能够促进叶绿素合成,浓度越大,促进作用反而降低。通过透射电镜照片,可以发现,磁性氧化铁纳米颗粒进入了植物细胞,并在细胞壁、细胞间隙、线粒体等处均有出现,这也许可以解释为什么使用磁性氧化铁纳米颗粒浇灌过以后,植物生理参数的一系列变化的根源在于这些磁性氧化铁纳米颗粒进入了植物根部的细胞及其细胞器。本研究首次揭开了氧化铁纳米颗粒与植物(绿豆)作用的微观机制,从分子水平证实了氧化铁纳米颗粒对植物(绿豆)生理的影响,有助于揭示纳米材料生物效应研究中纳米材料生命周期的关键一环,为阐明纳米材料的生物安全性提供了一条新的途径。
Hong-Xuan Ren, Ling Liu, Chong Liu, Shi-Ying He, Jin Huang, Jun-Li Li, Yu Zhang, Xing-Jiu Huang, and Ning Gu J. Biomed. Nanotechnol. 7, 677-684 (2011)
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Effect of magnetic nanoparticles on the germination of Chinese mung bean seeds.
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Root activity in the presence of 9 nm and 18 nm magnetic nanoparticles of various concentrations
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Physiological parameters in the presence of 9 nm and 18 nm magnetic nanoparticles of various concentrations. A) Activity of catalase (CAT). B) Activity of peroxidase (POD). C) Activity of superoxide dismutase (SOD).
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Physiological parameters in the presence of 9 nm and 18 nm magnetic nanoparticles of various concentrations. A) Content of chlorophyl. B)
Content of soluble protein (SP). C) Content of malondialdehyde (MDA).
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TEM images of mitochondria in the absence (A) and presence (B) MNPs suspension.