在活细胞研究中，因光子对细胞和生物分子损害最小、所获结果可信，光学方法备受关注。要实现对单细胞的光学检测，需容积小、光学收集效率高、低噪声的检测器和低的背景光。作为生物光学检测的传感器，光纤探针的尺寸形状对提高光学收集效率至关重要。
在实现光纤探针可控制备的基础上，采用化学镀Ag和超声探针顶部开口的方法，制备了顶部具有金属孔洞的银膜包覆探针，以满足细胞检测的要求。基于不同工艺得到的银膜包覆探针开口率和开口尺寸，筛选出了较佳的工艺条件。解决了光泄漏和无孔洞探针光通过率低的问题，得到了较大通光率的光纤探针。
Significant attention is focused on the optics application in the studies of alive cell for its smaller injury on cell and credible result. To achieve the optic detection in individual cell, it is prerequisite for optical detector to be tiny volume, highly optical collection- efficiency, low noise and light background. As the biosensor of optic transfer and measurement, suitable profile of fiber probe is essential for improvement of optical collection- efficiency.
Based on the controllable preparation of pencil-like fiber probes , probes with silver coating and aperture at the top were fabricated with the help of chemical plating and ultrasonic, in order to avoid the light leakage and meet the need of individual cell detection. According to the yield of silvering probe with aperture at the top and the size of aperture, better process in different techniques were filtered out. The probes prepared in this way have higher luminous flux and the leaking of light was also solved preferably.
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