以生理相容的单硬脂酸甘油酯、硬脂酸作为固体脂质基材，以大豆油作为液态脂质基材，采用高压均质法制备VE纳米结构脂质载体(VE-NLC)。TEM照片显示粒子呈不规则球形，且粒径分布均匀。VE-NLC体系的Zeta电位负于45 mV，4℃保存560天后粒径基本保持不变，物理稳定性好。细胞实验结果表明：当VE-NLC稀释600倍时对HaCat细胞培养体系无毒性作用，且培养液中VE含量在孵育4ｈ后明显下降，24h后已减少90%以上,说明VE-NLC具有优良的释放性能和通透性，且对UVB照射的保护作用呈时间递增性。
VE-loaded nanostructured lipid carriers (VE-NLC) were produced by melt high pressure homogenization, consisting of biocompatible materials. The optimal production parameters of VE-NLC dispersion were investigated to obtain small particle size and narrow size distributions of VE-NLC for its application in topic administration and cosmetic fields. TEM micrograph showed VE-NLCs were all irregular spherical particles with a narrow size distribution. Cell experiment results showed that no cytotoxicity was observed when VE-NLC dispersion of 5% lipid concentration was diluted by 600-fold. Further, cellular uptake and penetration of VE were necessary for its protection against photodamages of HaCaT keratinocytes induced by UVB irradiation. This work suggests that incorporation of VE in nanostructured lipid carriers confers photoprotection against UVB-induced photodamages.
Reference:
QUANHONG Ma, YIWU Wang, XIANGFEI Lin, DAN Luo,NING Gu. Preparation, Characterization and Photoprotection of Tocopherol Loaded Nanostructured Lipid Carriers. 2007 IEEE/ICME International conference on complex medical engineering-CME， 2007. 5，204-209
